Analysis Note

ControlPositive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.

Application

Marker for detection of receptor mediated endocytosis

Suitable for frozen sections

Western blotting KNOW SPECIES

Optimal working dilutions must be determined by the end user.

Research CategoryEpitope Tags & General Use

Research Sub CategoryOrganelle & Cell Markers

Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Coated vesicles (clathrin) of bovine brain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

The antibody was purified by gel filtration and is presented as 100µg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.

Format: Purified

Specificity

This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt.